Mycoplasma

1-wall-less, no growth on the lab media, no gram stain.
2-naturally resist to all antibiotics which stop cell wall synthesis.
3-all Mycoplasma is the smallest bacteria to grow on special lab media (in-vetro).
Medium: Mycoplasma salt medium or hypertonic salt cholesterol medium.
Temp: at 37c.
Colony appearance: fried egg colonies.
4-all Mycoplasma survives within human cells only.

Mycoplasma pneumoniae
Reservoir: human.
Transmission: droplet inhalation.
Disease: primary atypical pneumonia or walking pneumonia.
Lab diagnosis:
1-Specimen: sputum (very scanty).
2-Gram reaction: no gram stain because there is no cell wall.
3-Media: Mycoplasma salt mediafried egg colonies. Put around the colonies glucose-phenol red yellow color will appear due to microorganism produces acid from glucose, other microorganisms cannot.
4-serology:
A) Cold agglutinin: serum (Antibody) + human RBCs group O (Antigen).
Preserve at 4 c. not specific test.
b) Direct immunofluorescence.
*note: specimen: blood culture as confirmatory test.
Treatment:
Tetracycline and erythromycin.


Mycoplasma huminis
-Normal flora under the normal condition.
-arginine degradation, no acid from glucose.
-disease: intrapelvis disease.


Ureaplasma urealyticum
Reservoir: human.
Transmission: sexual transmitted disease (STD).
Disease: non Gonococcal urethritis (NGU).
Specimen: urethral discharge on mycoplasma salt medium contains urea deep pink color.
-urease +ve.
Treatment:
Tetracycline and erythromycin (both partners must be treated).